Linh Tran about Amytracker 480 and Amytracker 680
"I have the opportunity to work with Amytracker dyes for my PhD project and find them to be exceptional. These dyes deliver superb brightness and an excellent signal-to-noise ratio, making them an outstanding tool for the fluorescent visualization of amyloid-beta. On tissue sections, the staining procedure is both simple and quick. Amytracker dyes complement other amyloid probes that I use, offering promising potential for gaining additional insights into amyloid pathology. In addition, technical consultation with Ebba team was pleasant and efficient, making the entire process a great experience."
Linh Tran, PhD student, Theodor Kocher Institute, Switzerland.
Manuela Leri about Amytracker 630
"I used the Amytracker 630 probe to visualize intracellular aggregates on cell cultures. I obtained excellent results using confocal microscopy. The cells were permeabilized and the probe recognized the primary antibody used very well and emitted a good signal. The signal is stable."
Manuela Leri (PhD) Postdoctoral Researcher, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Italy
Azad Farzadfard about Amytracker 580 and Amytracker 680
"Amytracker was a great substitute for ThT in visualizing the alpha-synuclein fibrils inside the water-in-oil emulsion droplets made in microfluidic devices. ThT leakage from these droplets was an issue that was resolved by Amytracker products. I started by using Amytracker 480 that was a great upgrade for my experiments in compare to ThT. No leakage and footprint on PDMS was observed, but Amytracker 480 still showed background inside the droplets in my setup. Replacing it with Amytracker 680, however, removed the background completely with high sensitivity for the fibrils."
Azad Farzadfard (PhD) Postdoctoral Researcher, Department of Biotechnology and Biomedicine Section for Protein Chemistry and Enzyme Technology, Technical University of Denmark
Tom Cornelissen, PhD, reMYND Science Director Contract Research:
“At reMYND's Contract Research Organization (CRO), we specialize in conducting efficacy and proof of concept studies using mouse models for Alzheimer's and Parkinson's disease. In our search for innovation, we have integrated Amytracker 520 from Ebba Biotech into our research protocols, and the results have been exceptional.
Amytracker 520 has significantly enhanced our ability to detect and analyse key pathological features in brain tissue. Specifically, we have successfully identified amyloid plaques, Tau tangles, Lewy body-like inclusions, and alpha-synuclein preformed fibrils (PFFs, which were administered stereotactically). The specificity of the stain, combined with a high signal-to-noise ratio, ensures that our findings are both accurate and reliable (find out more HERE).
Furthermore, the narrow spectrum of Amytracker 520 makes it an excellent choice for co-staining applications, allowing us to achieve comprehensive and detailed imaging results. This will be invaluable for our clients to advance the understanding of the underlying mechanisms of neurodegenerative diseases and in the development of potential therapeutic strategies.
Overall, Amytracker 520 has proven to become a critical tool in our research arsenal, and we highly recommend it to other researchers in the field of neurodegenerative disease studies."
Tom Cornelissen (PhD), Science Director Contract Research, reMYND, Leuven, Belgium
Prof. Fabrizio Chiti about Amytracker 630:
"I like Amytracker probes because they can be used to detect amyloid-like species inside cells. We have used Amytracker 630 to exclude amyloid-like species of TDP-43 expressed in NSC34 cultured cells. We have also used cells treated with BSA and preformed Abeta fibrils as negative and positive controls respectively, all internalised with a specific kit. We detected fluorescence only in the latter case, as expected. Cells were fixed, permeabilized with Triton X-100 and Amytracker 630 was then added."
Prof. Fabrizio Chiti is Full Professor of Biochemistry leading the Laboratory for the Study of Protein Misfolding Diseases at the University of Florence in Italy.
Adam Kreutzer about Amytracker 680:
“I have been very happy with the Amytracker dyes I have used thus far. I have easily worked the Amytracker dyes into my free-floating, fixed brain tissue immunostaining workflow. The nice thing about the Amytracker dyes is that I don’t have to dehydrate the tissue in a series of ethanols and xylene, which is required for the widely used thioflavin and congo red dyes. The Amytracker dyes can just be applied to the tissue in TBS and then imaged. I often don’t even wash the tissues after treatment with the Amytracker dyes."
Adam Kreutzer, PhD, Associate Project Scientist at the Department of Chemistry, Nowick Laboratory, University of California, Irvine, USA
Keiza Jack about Amytracker 540:
“I have used Amytracker 540 in my PhD project as a tool to measure the structural differences of prion structures and prion-seeded amyloid fibrils. Amytracker 540 reports sensitively on subtle structural differences between protein structures, giving me a fast and reproduceable method to compare protein structures, which was essential to investigate my thesis"
Kezia Jack from MRC Prion Unit, Institute of Prion Diseases, University College London, London, UK
Dr. Jaakko Sarparanta about Amytracker 680:
”We used Amytracker 680 to study the amyloid-like nature of pathological protein aggregates in muscle sections. The bright positive staining was easily interpreted and provided the much needed support for our Congo Red results.”
Dr. Jaakko Sarparanta, Folkhälsan Research Center, Helsinki, Finland.
M. Garcia about Amytracker 520:
"We are studying Alzheimer’s disease in mouse models and use a variety of anti-amyloid-beta antibodies and traditional dyes to look at amyloid-beta aggregation. Amytracker 520 gave a very clean staining with high signal to noise. It was easy to use as a part of routine immunohistochemistry and made for a great complement to Thioflavin S staining to detect dense-core plaques with much less background."
M. Garcia (MSc), Doctoral student, Sweden