A publication in iScience by Jitka Petrlova from Artur Schmidtchen’s group at Lunds University discovered an interesting connection between Lipopolysaccharides (LPS) and the wound healing process. The researchers found that LPS, the outer membrane component of gram-negative bacteria, can induce protein aggregation in wounds and isolated wound fluids. This process would, in turn, reduce inflammation and contribute to wound healing. To detect the formation of amyloid-like aggregates, they used Amytracker 680 and observed under a fluorescent microscope.
Do you want to learn more details about how Amytracker was used in this publication? Click here
Image: Protein aggregation in acute wound fluids (AWFs) in the presence and absence of LPS. Fluorescence microscopy images of AWFs +/- LPS (100 μg/ml) labeled with Amytracker 680 in red. An increase in the Amytracker 680 signal indicates aggregates in AWFs after the addition of LPS. Image from Figure 2D by Petrlova et al. (CC BY 4.0)