Amytracker are optotracers with structure-dependent photo-physical properties. All Amytracker variants are designed to bind to the Congo red binding pocket on the amyloid fibril and require a theoretical minimum of eight in-register parallel-β-strands for binding. Therefore, Amytracker reliably labels amyloids derived from a variety of amyloidogenic proteins or peptides from different species. Due to their structure-dependent photo physical properties, the Amytracker variants are only fluorescent when binding to a target and different targets can produce a difference in the molecules fluorescence spectrum. To investigate different targets, we recommend to perform imaging by exciting the sample with different wavelengths collecting fluorescence intensity in multiple emission ranges (see the table below for reference). Excitation- and emission spectra for all Amytracker variants can be accessed here.
| Channel | Excitation | Emission range | Amytracker variant |
|---|---|---|---|
| CH1 | 405 nm | 400-490 nm | Amytracker 480 Amytracker 680 |
| CH2 | 405 nm | 490-600 nm | Amytracker 480 Amytracker 680 |
| CH3 | 405 nm | 600-660 nm | Amytracker 480 Amytracker 680 |
| CH4 | 405 nm | 660-700 nm | Amytracker 480 Amytracker 680 |
| CH5 | 488 nm | 500-580 nm | Amytracker 520 Amytracker 540 Amytracker 630 |
| CH6 | 488 nm | 580-650 nm | Amytracker 520 Amytracker 540 Amytracker 630 |
| CH7 | 488 nm | 650-700 nm | Amytracker 520 Amytracker 540 Amytracker 630 |
| CH8 | 561 nm | 600-650 nm | Amytracker 630 Amytracker 680 |
| CH9 | 561 nm | 650-700 nm | Amytracker 630 Amytracker 680 |
| CH10 | Brightfield | ||